This article presents a study on the antibiotic-producing capability of Actinomycetes strain ST-13-2, utilizing the bioautograph technique to assess antibacterial activity. The findings revealed that the produced compounds are water-soluble basic antibiotics with a clear inhibitory effect against Staphylococcus aureus. Some of the substances exhibited more than one inhibition zone, and one of the zones had an Rf value equivalent to that of Cloxacillin (Rf = 0.26), indicating potential antimicrobial properties.
Further testing demonstrated that the antibiotic substances from this strain were capable of inhibiting the growth of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae, based on 30 clinical isolates obtained from hospitalized patients. The results showed that all bacterial strains were inhibited, except for Pseudomonas aeruginosa, which exhibited only partial susceptibility.
Iron fortification trial was undertaken for a duration of two years in Amphoe Uthuтphon Phisai, Changwat Si Sa Ket. A total population of 4,242 in 6 villages was included in the study and a group of females age 15-45 years of which the subgroups were 124 placebo, 129 fortified salt and 165 combined fortified salt and fortified fish sauce, was selected for paired study.
The detection of haptoglobin in bloodstains is carried out by extracted blood in tris-citrate buffer and run in starch gel electrophoresis in NaOH-boric and stained with benzidine-hydrogen peroxide. The persistence of haptoglobin varies according to the supporting medium and environment (heat, moisture) and also to its phenotypes. The duration for detection of haptoglobin in bloodstains is between 1 and 7 days.
Suspension of hepatocytes was isolated from the livers of male Wistar rats weighing 200-250 g. by using the method of Berry and Friend as modified by Stacey and Priestly. Morphological features, plasma membrane integrity and metabolic capability were used as the criterion for cell viability. The successful method to obtain good cell preparations must involve three critical steps ; namely, exposure of the liver to a calcium free medium, digestion with collagenase in a recirculating system and gentle mechanical treatment. Albumin (1.2%) in the final medium which was used for suspending cells is necessary to maintain cell viability. Cell yields of 2-3 × 10 8 cells were obtained with a trypan blue exclusion index of 90-98%. Cell preparations with a trypan blue exclusion index of less than 90% were not recommended for any studies.
