{
    "url": "https://mhesi.nrct.go.th/archives/s/mhesi_archives/item/6412",
    "o:resource_class": "dctype:Text",
    "dcterms:title": [
        "Isolated liver cell preparations"
    ],
    "dcterms:creator": [
        "Pornpen Pramyothin"
    ],
    "dcterms:subject": [
        "Liver cells"
    ],
    "dcterms:description": [
        "Suspension of hepatocytes was isolated from the livers of male Wistar rats weighing 200-250 g. by using the method of Berry and Friend as modified by Stacey and Priestly. Morphological features, plasma membrane integrity and metabolic capability were used as the criterion for cell viability. The successful method to obtain good cell preparations must involve three critical steps ; namely, exposure of the liver to a calcium free medium, digestion with collagenase in a recirculating system and gentle mechanical treatment. Albumin (1.2%) in the final medium which was used for suspending cells is necessary to maintain cell viability. Cell yields of 2-3 × 10 8 cells were obtained with a trypan blue exclusion index of 90-98%. Cell preparations with a trypan blue exclusion index of less than 90% were not recommended for any studies."
    ],
    "dcterms:publisher": [
        "National Research Council Thailand"
    ],
    "dcterms:date": [
        "1986"
    ],
    "dcterms:type": [
        "Text"
    ],
    "dcterms:format": [
        "pdf"
    ],
    "dcterms:source": [
        "Journal of National Research Council, Vol. 18, No. 1 January-June 1986 , 1-9",
        "Library and Information Center, National Institute of Development Administration"
    ],
    "dcterms:language": [
        "en"
    ],
    "dcterms:coverage": [
        "สื่อและเอกสารเผยแพร่"
    ],
    "dcterms:rights": [
        "This is an open access document. You are free to Share and Use under the terms of the Creative commons license."
    ],
    "dcterms:alternative": [
        "เซลล์ตับอิสระ"
    ],
    "dcterms:created": [
        "2025-05-29"
    ],
    "dcterms:extent": [
        "9 leaves, 5.45 MB"
    ],
    "dcterms:accessRights": [
        "This document is distributed under the terms of the Creative Commons Attribution 4.0 International (CC-BY-NC-ND 4.0) and copyright of the Chulachomklao Royal Military Academy"
    ],
    "dcterms:license": [
        "This document is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License."
    ],
    "dcterms:rightsHolder": [
        "Ministry of Higher Education, Science, Research and Innovation"
    ]
},