Url
	https://mhesi.nrct.go.th/archives/s/mhesi_archives/item/6412
Resource class
	dctype:Text
Title
	Isolated liver cell preparations
Creator
	Pornpen Pramyothin
Subject
	Liver cells
Description
	Suspension of hepatocytes was isolated from the livers of male Wistar rats weighing 200-250 g. by using the method of Berry and Friend as modified by Stacey and Priestly. Morphological features, plasma membrane integrity and metabolic capability were used as the criterion for cell viability. The successful method to obtain good cell preparations must involve three critical steps ; namely, exposure of the liver to a calcium free medium, digestion with collagenase in a recirculating system and gentle mechanical treatment. Albumin (1.2%) in the final medium which was used for suspending cells is necessary to maintain cell viability. Cell yields of 2-3 × 10 8 cells were obtained with a trypan blue exclusion index of 90-98%. Cell preparations with a trypan blue exclusion index of less than 90% were not recommended for any studies.
Publisher
	National Research Council Thailand
Date
	1986
Type
	Text
Format
	pdf
Source
	Journal of National Research Council, Vol. 18, No. 1 January-June 1986 , 1-9
	Library and Information Center, National Institute of Development Administration
Language
	en
Coverage
	สื่อและเอกสารเผยแพร่
Rights
	This is an open access document. You are free to Share and Use under the terms of the Creative commons license.
Alternative Title
	เซลล์ตับอิสระ
Date Created
	2025-05-29
Extent
	9 leaves, 5.45 MB
Access Rights
	This document is distributed under the terms of the Creative Commons Attribution 4.0 International (CC-BY-NC-ND 4.0) and copyright of the Chulachomklao Royal Military Academy
License
	This document is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Rights Holder
	Ministry of Higher Education, Science, Research and Innovation

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