Brine shrimp (Artemia sp.) culture experiment in waste water from shrimp farming was operated for 37 days without water circulation and aeration supply. Six circular concrete tanks of 350 l were used. The prior period of the experiment was designed without water exchange to study the difference in water quality between two treatments. The one was control, designed without Artemia, and the other one was stocked at a density of 100 individuals/l (pre-adult stage). Three replications were conducted for each treatment. The results showed the significant (p<0.05) difference in pH, nitrite, nitrate, ammonia, orthophosphate, total phosphate, dissolved oxygen, biological oxygen demand (BOD), chlorophyll a, chlorophyll b and chlorophyll c content.